Journal article
Direct repression of MYB by ZEB1 suppresses proliferation and epithelial gene expression during epithelial-to-mesenchymal transition of breast cancer cells
HJ Hugo, L Pereira, R Suryadinata, Y Drabsch, TJ Gonda, NPAD Gunasinghe, C Pinto, ETL Soo, BJW van Denderen, P Hill, RG Ramsay, B Sarcevic, DF Newgreen, EW Thompson
Breast Cancer Research | BMC | Published : 2013
DOI: 10.1186/bcr3580
Abstract
Introduction: Epithelial-to-mesenchymal transition (EMT) promotes cell migration and is important in metastasis. Cellular proliferation is often downregulated during EMT, and the reverse transition (MET) in metastases appears to be required for restoration of proliferation in secondary tumors. We studied the interplay between EMT and proliferation control by MYB in breast cancer cells.Methods: MYB, ZEB1, and CDH1 expression levels were manipulated by lentiviral small-hairpin RNA (shRNA)-mediated knockdown/overexpression, and verified with Western blotting, immunocytochemistry, and qRT-PCR. Proliferation was assessed with bromodeoxyuridine pulse labeling and flow cytometry, and sulforhodamine..
View full abstractRelated Projects (2)
Grants
Funding Acknowledgements
HJH was supported by a National Breast Cancer Foundation Postdoctoral Training Fellowship; and EWT and HJH were supported also by the Cancer Council of Victoria; NPADG was supported by The Australian Government's Endeavour Awards Scholarship Program; BvD and ETLS were supported by the National Breast Cancer Foundation (Australia) and Cancer Australia; EWT, DFN, TJG and CP are active members of the EMPathy Breast Cancer Network, a National Breast Cancer Foundation (Australia)-funded National Collaborative Research Program. This study benefited from support by the Victorian Government's Operational Infrastructure Support Program to St. Vincent's Institute and Murdoch Children's Research Institute.